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KMID : 0357419940240010063
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Korean journal of Virology
1994 Volume.24 No. 1 p.63 ~ p.70
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Quantitation of HIV p24 Antigen Positive CD4+ Cell using Flow Cytometry
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Abstract
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identification of factors that correlate with progression of human immunodificiency virus (HIV)infection is important for prognosis and monitoring of therapy. We tried flow cytometric method for detection of HIV p24 antigen in CD4+cells.
Lymphocytes
harvested from HIV infected persons were treated with a methanoliaceton fixative solution for giving permeability and followed by binding of monoclonal antibody specific for HIV p24 antigen. After labeling with a fluorescein
isothiocyanate-conjugated
F(ab')2 fragments of goat anti-mouse immunoglobulin antibody, p24 antigen positive CD4+ cells were counted using flow cytometry, We followed 40 HIV infected persons during the peried of Sept. 1992 and Sept. 1993. The proportion of p24 antigention
of
CD4+ cells(Y) 3 months before and after the test (Y=-4.59X+44.45). On the other hand there was less correlation [r=-0.356(p=0.046)]with absolute CD4+lymphocyte count which was directly linked to clinical stage. This results suggest that flow
cytometry
can be used as a rapid, sensitive and quantitative assay system for detection of viral replication status of HIV-seropositive persons and it could be the most accureat prognostic marker for HIV disease.
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KEYWORD
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